midashi

  • 1  siRNA配列設計法

    Kumiko Ui-Tei, Yuki Naito, and Kaoru Saigo Essential Notes Regarding the Design of Functional siRNAs for Efficient Mammalian RNAi ,Journal of Biomedicine and Biotechnology Volume 2006 (2006)

    こちらよりダウンロード可能です。

    Yuki Naito, Tomoyuki Yamada, Kumiko Ui-Tei, Shinichi Morishita and Kaoru Saigo siDirect: highly effective, target-specific siRNA design software for mammalian RNA interference Nucleic Acids Res., Jul 2004; 32: W124 - W129.

    こちら よりダウンロード可能です。

    Tomoyuki Yamada and Shinichi Morishita Accelerated off-target search algorithm for siRNA, Bioinformatics, 21(8):1316-1324, 2005

    こちら よりダウンロード可能です。


  • 2 フォーク型RNAi

    Hohjoh H. (2004) Enhancement of RNAi activity by improved siRNA duplexes. FEBS letters, 557: 193-198.

    こちら よりダウンロード可能です。

    Ohnishi Y., Tokunaga K., and Hohjoh H. (2005) Influence of assembly of siRNA elements into RNA-induced silencing complex by fork-siRNA duplex carrying nucleotide mismatches at the 3'- or 5'-end of the sense-stranded siRNA element. BBRC, 329: 516-521

  • 3 キメラ型RNAi

    Kumiko Ui-Tei1,*, Yuki Naito1, Shuhei Zenno1, Kenji Nishi1, Kenji Yamato2, Fumitaka Takahashi1, Aya Juni1 and Kaoru Saigo1
    Functional dissection of siRNA sequence by systematic DNA substitution: modified siRNA with a DNA seed arm is a powerful tool for mammalian gene silencing with significantly reduced off-target effect

    こちら よりダウンロード可能です。


  • 4 siRNA mini-libraryを用いて同定したAP2alphaの解析

    Yukiko Kitagawa, Masanori Kameoka, Sanae Shoji-Kawata, Yukie Iwabu, Hiroyuki Mizuta, Kenzo Tokunaga, Masato Fujino, Yukikazu Natori, Yoshiaki Yura, Kazuyoshi Ikuta
    Inhibitory function of adapter-related protein complex 2 alpha 1 subunit in the process of nuclear translocation of human immunodeficiency virus type 1 genome

    こちら よりダウンロード可能です。